Matherville is a village in Mercer County, Illinois, United States. The population was 772 at the 2000 census.Matherville is located at 41°15′37″N 90°36′21″W / 41.26028°N 90.60583°W / 41.26028; -90.60583.According to the 2010 census, Matherville has a total area of 0.398 square miles, of which 0.39 square miles is land and 0.008 square miles is water.As of the census of 2000, there were 772 people, 290 households, and 216 families residing in the village. The population density was 1,968.7 people per square mile. There were 301 housing units at an average density of 767.6 per square mile. The racial makeup of the village was 98.45% White, 0.26% Asian, 0.52% from other races, and 0.78% from two or more races. Hispanic or Latino of any race were 1.04% of the population. - - There were 290 households out of which 45.2% had children under the age of 18 living with them, 55.5% were married couples living together, 15.2% had a female householder with no husband present, and 25.